International Journal of Botany and Horticulture Research(IJBHR)
ISSN: 2994-872X | DOI: 10.33140/IJBHR
Impact Factor: 0.98
Jieping Li
International Potato Center (CIP), China Center for Asia-Pacific (CCCAP), China
Publications
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Research Article
A Simple and Efficient Agrobacterium Tumefaciens-Mediated Transgenic System for Tetraploid Potato Cultivar Desiree
Author(s): Chunjiang Xu*, Dong Wang, Lipan Qi, Shiwei Chang, Pingping Yuan, Zhenxin Zhang, Youjiao Shan, Eric Magembe, Philip Kear, Yan Feng, Yafei Li, Yihong Ji, Lei Wang, Yan Feng and Jieping Li
As the fourth staple food crop in the world, the potato has the potential to provide adequate amounts of energy, protein, and nutrients necessary for humanity. However, given the rapid expansion of the global population and the adverse impacts of climate change, there is an urgent need for enhanced yields and enduring resilience. This study addresses this need by presenting a simple and efficient transgenic system for the tetraploid potato cultivar Desiree. The system, built by testing different potato materials, explants types, selection medium, selection agents, and Agrobacterium tumefaciens EHA105 strain carried different vectors, stands out for its remarkable speed, producing positively transformed seedlings in about 40 days. Compared to some traditional methods with the pre-treatment of explants and two-step regeneration stages, which need more operations and time, in this system.. Read More»