A Simple and Efficient Agrobacterium Tumefaciens-Mediated Transgenic System for Tetraploid Potato Cultivar Desiree

Abstract

Chunjiang Xu, Dong Wang, Lipan Qi, Shiwei Chang, Pingping Yuan, Zhenxin Zhang, Youjiao Shan, Eric Magembe, Philip Kear, Yan Feng, Yafei Li, Yihong Ji, Lei Wang, Yan Feng and Jieping Li

As the fourth staple food crop in the world, the potato has the potential to provide adequate amounts of energy, protein, and nutrients necessary for humanity. However, given the rapid expansion of the global population and the adverse impacts of climate change, there is an urgent need for enhanced yields and enduring resilience. This study addresses this need by presenting a simple and efficient transgenic system for the tetraploid potato cultivar Desiree. The system, built by testing different potato materials, explants types, selection medium, selection agents, and Agrobacterium tumefaciens EHA105 strain carried different vectors, stands out for its remarkable speed, producing positively transformed seedlings in about 40 days. Compared to some traditional methods with the pre-treatment of explants and two-step regeneration stages, which need more operations and time, in this system explants were directly inoculated with A. tumefaciens, a single-regeneration medium (SEG or SE) were used, which decreased operations and saved time. Leaf disc explants are eligible. The concentration of 20 μg/L is suitable for chlorsulfuron selection. The positive transgenic efficiency of tetraploid recipient Desiree is up to 68.79 %. Meanwhile, compare to diploid materials receptor CIP703541 and CIP705079, tetraploid materials Deisree have a lower ploidy change ratio after tissue culture process. This simple and fast transgenic system not only supports basic research on potato functional genes but also creates new potato materials for genetic engineering breeding, thereby contributing to the development of more resilient and productive potato varieties

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