Tephrosia Purpurea Modulates Hepatic Markers and Antioxidant Enzymes Against Two-Step Hepatocarcinogenesis Induced by N-Nitrosodiethylamine and Carbon Tetrachloride in Swiss Albino Rats

Abstract

Ramaswami Kartik*, G.D Reddy, Ch.V Rao, S.P Trivedi and P.Pushpangadan

Tephrosia purpurea (Leguminosae) has been used as hepatoprotective and is used to treat liver disorders. The study was aimed to investigate the amelioration effect of T. purpurea extract (TPE) against two-step hepatocarcinogenesis induced by N-nitros- odiethylamine (NDEA) and tumor promoter, carbon tetrachloride (CCl4) in Swiss albino rats and the mechanism of its hepa- tocarcinogenesis suppression effect. The hepatic function markers viz. SGPT, SGOT, SALP, gamma glutamyl transpeptidase (GGT), bilirubin, Protein and antioxidant enzymes viz. catalase, superoxide dismutase, reduced glutathione, glutathione peroxi- dase and glutathione-s-transferase, lipid peroxidation (LPO) were assayed. The hematological and nucleic acid viz. WBC, RBC, Hb, DNA and RNA were also assayed. Administration of TPE effectively suppressed hepatic marker and oxidative stress marker as revealed by decrease in NDEA and CCl4 induced elevated levels in hepatic marker, WBC and LPO and significant elevation in the level of antioxidant enzymes, protein, Hb and RBC. The body weight, mean and relative liver weight were assessed. The histopathology of liver revealed that TPE reduced the incidence of liver lesions. These results suggest that an extract of Tephro- sia purpurea is able to alleviate the hepatic and oxidative stress markers, which signify its amelioration effect against two-step NDEA and CCl4-induced hepatocarcinogenesis in swiss albino rats.

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