Combined Effect of Zingiber Officinale Rhizomes Extracts and Dried Fruits of Tetrapleura Tetraptera on Attenuated Monosodium-Iodoacetate-Induced Osteoarthritis Via its Analgesic, Anti-Inflammatory, Antioxidant and Anxiolytic Effects

Abstract

Djami Bejoutance Marcelle, Bilanda Danielle Claude, Atsamo Albert Donatien, Yousseu Nana William, Bomba Tatsinkou Francis Desire, Djuichou Nguemnang Stephanie Flore, Tcheutchoua Yannick Carlos, Bakouatche Jovanie Destin, Kwenthe Ernest Allah Hoki, Kana Miariam Flore, Mbiantcha Marius, Ateufack Gilbert and Dzeufiet Djomeni Paul

Ethnopharmacological Relevance: Zingiber officinale and Tetrapleura tetraptera are used by the population to treat many diseases.

Aim of the Study: This study aimed to evaluate the therapeutic effects of the mixture of dried fruits of Tetrapleura tetraptera extract and the rhizomes of Zingiber officinal extract on osteoarthritis.

Materials and Methods: Osteoarthritis was induced with monosodium-iodoacetate (50 μl, 100 mg/kg, i.a.). The extracts of the mixture of Zingiber officinale and Tetrapleura tetraptera (100, 200 mg/kg) was administered orally. Body mass, thermal hyperalgesia, paw volume, locomotion and anxiety states were assessed. The hematological, biochemical, anti- inflammatory, pro-inflammatory and oxidative stress parameters were measured. The microarchitecture of the knee joint was evaluated.

Results: Extracts (200 mg/kg) have reduced hypersensitivity (69.33%; 79.03%); and also, the paw volume (63.96%; 81.85%). The extracts improved the locomotion and reduced the total time of immobility. Extracts have increased the number of lines crossed and the mobility time episode and have improved the oxidative status of animals. It has inhibited TNF-α, IL-1β, MMP9, MPO, and stimulated IL-4, IL-10. Extracts have ameliorated histopathological alterations in the knee joint.

Conclusions: Results show antihyperalgesia and anti-inflammatory properties of the mixture of Zingiber officinale and Tetrapleura tetraptera, and is able to improve locomotion damage and anxiety status caused by osteoarthritis.

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