Yurii Lesniak
I.D. Stem Cells and Genome Institute, Ukraine
Publications
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Review Article
In Vitro Differentiation of Bone Marrow Mesenchymal Stem Cells into Human Male Gametes
Author(s): Islam Al Dababsekh*, Yurii Lesniak, Natalia Oleksenko and Amina Al Dababsekh
Introduction: Male infertility is a growing global health concern, contributing to nearly half of all infertility cases, with conditions such as azoospermia presenting significant therapeutic challenges. Conventional treatments offer limited solutions, particularly for absolute infertility. The emergence of regenerative medicine, especially mesenchymal stem cell (MSC) therapy, offers new possibilities. This study investigates the feasibility of differentiating human bone marrow-derived MSCs into functional spermatocytes in vitro, presenting a potential alternative for patients lacking viable gametes. Materials and Methods: MSCs were mobilized using granulocyte colony-stimulating factor (G-CSF), isolated from peripheral blood, and cultured under a simulated germline environment. Key markers such as Oct-4, DAZL, and DMC1 were used to.. Read More»