Journal of Veterinary Health Science(JVHS)

Department of Epidemiology and Health Statistics, Dalian Medical University, 9 Lvshun South Road, Dalian, 116044, PR, China

Publications

• Research Article   
  Curcumin Induces MCF-7 Cells Pyroptosis via Autophagy/CTSB/NLRP3/ Caspase-1/GSDMD Signaling Pathway in Vitro and Vivo
  Author(s): Haonan Duan, Rufei Ma, Liping Jiang, Yue Du, Shu Ou, Xiuliang Guan, Nan Teng and Xiaofeng Li*
  
  Background: Curcumin, as a lipid-lowering drug, has been reported to be effective in the treatment of breast cancer. However, the underlying molecular mechanisms have not been completely investigated. Methods: MTT assay was used to determine the effect of curcumin on survival rate of MCF-7 cells. The effects of curcumin on tumor growth were observed in animal models of breast cancer. The positive reactions of Caspase-1, IL-1β and IL-18 were detected by immunohistochemistry. LC3, p62, CTSB, ASC, Pro-Caspase-1, GSDMD, NLRP3, Caspase-1, GSDMD-N, IL-1β and IL-18 were determined by Western blot in vitro and vivo. ELISA determined the release of extracellular IL-1β and IL-18. LDH release was measured. The expression level of CTSB in cytoplasm were determined by immunofluorescence assay. Cell proliferation, cell migration and tube fo.. Read More»
  

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