Advancement in Dairy Science Research(ADSR)
Tsion Bilata
Addis Ababa University, College of Veterinary Medicine and Agriculture, Department of Microbiology, Immunology and Veterinary Public Health, Sebeta Animal Health Institute, Ethiopia
Publications
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Research Article
Production of Polyclonal Antibodies (IgY) Against Foot-and-Mouth Disease (Serotype: O, A and Sat-2) in Ethiopia by Using Layer Hens
Author(s): Ephrem Shimelis*, Shimels Tesfaye, Fanos Tadesse, Tsion Bilata and Jan Paeshuyse
The use of chickens as experimental animals in laboratory for polyclonal antibody (IgY) production has increased in recent times, as its specificities to mammalian antigen, animal welfare (non-invasively extracted from egg yolk), high antibody per ml, very low cost and short turnover of production. The present study was aimed to produce IgY polyclonal antibody against the three Foot-and-Mouth disease viruses’ serotypes (O, A and SAT2) in Ethiopia by immunization of laying hens with the locally inactivated the same serotypes of Foot-and-Mouth disease viruses. At day 14, 21 and 28 post immunization, the eggs and serum were collected and the IgY polyclonal antibodies were extracted from the chicken egg yolk by using Polyethylene Glycol (PEG 6000g) precipitation method. The presence of the IgY polyclonal antibodies against the selected Foot-and- Mouth disease viruses’ serotype.. Read More»