International Journal of Cancer Research & Therapy(IJCRT)
ISSN: 2476-2377 | DOI: 10.33140/IJCRT
Impact Factor: 1.3
Jakob Sidenius Johansen
Steno Diabetes Center Aarhus, Aarhus University Hospital, Palle Juul-Jensens Boulevard, 99 indgang G plan 2 G208, 8200 Aarhus, Denmark
Publications
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Research Article
Technical and Biological Variations in the Purification of Extrachromosomal Circular DNA (eccDNA) and the Finding of More eccDNA in the Plasma of Lung Adenocarcinoma Patients Compared with Healthy Donors
Author(s): Egija Zole, Lasse Bollehuus Hansen, Janos Hasko, Daniela Gerovska, Marcos J. Arauzo-Bravo, Julie Boertmann Noer, Yonglun Luo, Jakob Sidenius Johansen and Birgitte Regenberg*
Human plasma DNA originates from all tissues and organs, and has a potential to act as a versatile marker for diseases such as cancer, since fragments of cancer-specific alleles can be found circulating in the blood. While linear DNA has been studied intensely as a liquid biomarker, the role of circular circulating DNA in cancer is more unknown due, in part, to a lack of comprehensive testing methods. Our developed method profiles extrachromosomal circular DNA (eccDNA) in plasma, integrating Solid-Phase Reversible Immobilization (SPRI) bead purification, the removal of linear DNA and mitochondrial DNA, and DNA sequencing. As an initial assessment, we tested the method, biological variations, and technical variations using plasma samples from four patients with lung adenocarcinoma and four healthy and physically fit individuals. Despite the small sample group, we observed a significant.. Read More»