I. K. M. Okorie
Department of Microbiology, University of Ibadan, Nigeria
Publications
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Research Article
Characterisation of a novel naturally-modified biogenic calcium carbide-containing exopolysaccharide produced from Pseudomonas aeruginosa strain S16 from soil in Nigeria, West Africa
Author(s): I. K. M. Okorie* and A. A. Ogunjobi
Antibiotic resistance, that occurs globally, has led to a reduction in the efficacy of many contemporary conventional antibiotics. The aim of this study was to characterise an exopolysaccharide, produced from Pseudomonas aeruginosa strain S16, accession number OQ734845, that was observed to be effective against two antibiotic-resistant strains, namely Klebsiella pneumoniae and Staphylococcus aureus SO183, in another study. HPLC and MS of the exopolysaccharide, confirmed the presence of glucose, rhamnose, xylose arabinose, D-ribose, galactose, mannose and inositol. The phenol-sulfuric acid method, quantified the carbohydrate sugars concentration, as 0.0060 g/L. SEM showed that the exopolysaccharide possessed a sparsely flaky surface, with globular projections. EDX detected nitrogen (48.00%); carbon (20.10%); phosphorus (12.6%); sodium (8.00%); oxygen (6.00%); calcium (2.30%); chlorine .. Read More»
