Asit Kumar Chakraborty
Department of Biotechnology and Biochemistry, Oriental Institute of Science and Technology-West Beng, India
Publications
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Research Article
The Unique Deletion, Insertion and Point Mutation in The Upstream of Nucleocapsid (N) Gene of Different Covid-19 Variants Suggests Differential Translational Mechanisms of N-Protein
Author(s): Asit Kumar Chakraborty*
The nucleocapsid protein (N protein; 219 AAs) of SARS-CoV-2 was synthesized from terminal part of the ~30kb genome and a 31ERS three AAs deletion was reported in all Omicron variants but not in Alpha, Beta, Gamma and Delta variants. We found that upstream of N-gene which also a part of ORF8 gene has different deletion, insertion and point mutations in different corona virus lineages. As for example, 119DF two amino acids (5’-28243GATTTC-3’) deletion was found in ORF8 protein of Delta variants located adjacent to the N-gene upstream. Similarly, four nucleotides (5’-AACA-3’) insertion at 28262 position was found in the Gamma (P.1) variant only. Whereas all Omicron variants (BA.1/BA.2/BA.4/ BA.5) and subvariants (XBB.1.5, BQ.1.1) which were spreading now with mild diseases, have no such deletion or inser- tion. Some point mutations T>C at 28247 position were fo.. Read More»