Green Synthesis Characterization and Biological Assessment of Nickle based Microparticles using Justicia Adathoda L. Leaf Extract

Abstract

Waqas Ahmad*, Aftab Alam*, Noor Ul Islam*, Abdul Wahab, Muhammad Ishaq Khan, Wajid Ali and Abass Khan

Nickel-based oxide/hydroxide microparticles (NiO/Ni(OH)2 MPs) were synthesized via a green way utilizing Justicia adathoda L. leaf extract, serving as novel reducing and stabilizing agent. Plants phytochemicals such as alkaloids, flavonoids, and phenolics facilitated the eco-friendly formations of NiO/Ni(OH)2 MPs without toxic chemicals. Structure analysis through X-ray diffraction (XRD) confirmed the crystalline structure, while FTIR confirmed the involvement of functional groups in reduction and stabilizations. Morphological characterization by Scanning Electron Microscopy (SEM) revealed a granular dense structure microparticle of size about 1μm. Biological screening showed notable antibacterial and antifungal activities. NiO/Ni(OH)2 MPs show 60.24% inhibition against Staphylococcus aureus and 70.24% inhibition against Pseudomonas aeruginosa, while antifungal activity reached 95% against Aspergillus fumigatus. Compared to standard pharmaceutical agents such as Ofloxacin and Miconazole, the NiO/Ni(OH)2 MPs synthesized using Justicia adathoda L. leaf extract revealed potential antibacterial and antifungal activities. These results underline the value of Justicia adathoda as an eco-friendly and efficient biological resource for making functional NiO/Ni(OH)2 MPs. The synthesized microparticles show strong applications in biomedical fields, including therapeutic formulations, antimicrobial coatings, and presenting a sustainable unconventional to chemically synthesized micromaterials.

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