Journal of Future Medicine and Healthcare Innovation(JFMHI)
ISSN: 3065-7628 | DOI: 10.33140/JFMHI
Srimanth Kumar Barigela
Department of Genetics and molecular medicine, Kamineni hospitals, Hyderabad, India
Publications
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Research Article
Pharmacogenomics in Critical Care: Advancing Precision Medicine for Optimized Drug Therapy and Improved Patient Outcomes
Author(s): Gulfiza Qadir*, Srikanth Jilla, Srimanth Kumar Barigela, Venkata Ramana K, Medavaram Sringala Devijan, B Kishan Sing Naik, Sreelatha Komandur and Qurratulain Hasan
Background Patients in the critical care unit often have complex, acute medical conditions requiring multiple drug regimens. Therapy relies on clinical guidelines and physician experience. This preliminary study evaluated functional polymorphisms in six genes affecting metabolism of drugs often used in ICU patients. Methods Blood samples from patients of Intensive Care Unit (ICU), Kamineni Hospitals, Hyderabad, India were collected over one month. Genomic DNA was extracted as done routinely for diagnostics. PCR was carried out for a functional variant in six pharmacologically relevant genes—ABCB1, CYP2B6, CYP2C9, CYP2C19, CYP3A4, and CYP3A5. The PCR products were analysed by Restriction Fragment Length Polymorphism (RFLP) or Sanger sequencing. The genotypes identified were categorized as normal, intermediate and poor metabolizers. .. Read More»