Shahrokh Shirazi
Department of Pathobiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Aza, Iran
Publications
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Research Article
The Diagnosis of Sheep Cystic Echinococcosis by Native Antigen B Elisa Method
Author(s): Shahrokh Shirazi, Nasser Hoghooghi-Rad, Rasoul Madani* and Fariba Golchinfar
Cystic echinococcosis (CE) is one of the most prevalent zoonotic diseases in some countries in the world. Cystic echinococcosis is considered a neglected disease. This disease increases economic damage via medical costs and loss of human and livestock productivity. The aim of the current study gains a better understanding of the prevalence of CE in sheep. Totally 250 sheep sera were collected. 25 serum samples from newborn lambs were used as negative, and 25 others were obtained from slaughterhouses as positive to infection with CE and 200 unknown sera. Antigen B Isolated from hydatid cysts fluid was used for designing ELISA methods. The first Method used anti-Sheep conjugate (SIGMA, at 1:3000 dilution). According to the results, the seroprevalence of CE in East Azerbaijan of Iran was 5.5% and sensitivity and specificity for the diagnosis of hydatidosis in sheep by AgB-ELISA methods w.. Read More»