Robertson P
College of Dental Medicine, Roseman University of Health Sciences, South Jordan, UT, USA
Publications
-
Research Article
RAGE Expression and Invasiveness of Oral Squamous Cell Carcinoma Cells Via Differential Expression of Matrix Remodeling Enzymes
Author(s): Clarke D, Yeates E, Davis G, Harward K, Robertson P, Licari FW, Winden DR, Reynolds PR and Arroyo JA*
Oral squamous cell carcinoma is an aggressive cancer that affects approximately 30,000 people annually. Tobacco use is known to induce diseases characterized by abnormal oral squamous cell carcinoma cells. The receptor for advanced glycation end-products is a surface receptor that is up-regulated by cigarette smoke in diverse tissue types, including oral squamous cell carcinoma cells. Our objective was to determine the receptor for advanced glycation end-products message and protein expression, cell invasiveness, and potential regulatory enzymes implicated in matrix remodeling during cigarette smoke extract exposure. Gingiva-derived Ca9-22 cells and tongue-derived Cal27 cells were cultured and exposed to 0.05% cigarette smoke extractfor 24 hours. Cell invasion was determined and cells were lysed for either quantitative polymerase chain reaction or western blot analysis. The .. Read More»