Mr. Alshad Seyyadali
Institute of Sciences, Department of Botany, Banaras Hindu University, Varanasi, 221005, India
Publications
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Research Article
Blue-Light Disinfection of Removable Dental Prostheses and Appliances: Mitigating the Limitations of Existing Reagents and Techniques
Author(s): Dr. Alka Shukla, Mr. Alshad Seyyadali, Mrs. Sonam Rastogi and Prof. Gopal Nath*
Biofilms on removable dental prostheses create reservoirs for opportunistic pathogens, increasing the risk of localized and systemic infections. Effective, biocompatible disinfection strategies are essential to maintain oral health and prosthesis integrity. This study evaluated the antimicrobial efficacy of 450 nanometer blue-light phototherapy against Veillonella parvula, an early colonizer and bridging species in oral biofilms, and assessed its safety on human peripheral blood mononuclear cells. Biofilms were developed on polystyrene microtiter plates and coverslips, while planktonic cultures were prepared from the same inoculum. Samples were exposed to blue light for 10, 15, 20, 25, and 30 minutes. Biofilm biomass was quantified using crystal violet staining, planktonic viability was assessed by colony-forming unit enumeration, and cell cytocompatibility was evaluated through trypa.. Read More»