Plant Biology Soil Health Journal(PBSHJ)

Department of Biotechnology, Nigerian Defence Academy, Kaduna, Nigeria

Publications

• Research Article   
  Characterization of Partially Purified Phospholipase A2 Enzyme of Naja Katiensis Venom
  Author(s): Hassan A U*, Egbe N E, Ugochukwu O O, Onuh K, Kereakede E and Umar S I
  
  Snake bite issues has been for long a neglected public health concern in Nigeria and other African countries, causing high number of human fatalities annually. It however remains a serious medical and economic problem. This research is aimed at the characterization of partially purified Phospholipase A2 (PLA2 ) enzyme of Naja katiensis venom. The enzyme is extracted using a two way purification step (Gel filitration using G-75 and ion exchange chromatography on CM- Sephadex). The molecular weight of the enzyme is determine using Sodium Dodecyl-Sulphate Electrophoresis (SDS- Page). The extracted enzyme kinetic parameters were also determine, after which its relative optimum affinity/activity were also determine with related to different ranges of temperature, pH, metal ions and salts. The partially purified PLA2 gave a total enzyme activity of 3.11 μmol/min and an estimated extrapol.. Read More»
  

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