International Journal of Cancer Research & Therapy(IJCRT)

Independent Researcher, USA

Publications

• Research Article   
  Computational Design of a De Novo Binder for Claudin-6 (CLDN6)
  Author(s): Jingxing Chen*
  
  Claudin-6 (CLDN6) is a tight-junction membrane protein normally expressed in embryonic tissues but abnormally activated in several cancers, where it contributes to tumor growth and metastasis. Its restricted oncofetal expression makes CLDN6 an attractive target for selective cancer therapies, yet designing molecules that bind its small, flexible extracellular loops has proven challenging. Here, we report the first de novo computational design of a protein binder to our knowledge that specifically engages the extracellular domain of human CLDN6. Using RFdiffusion, we guided backbone generation around a defined CLDN6 surface epitope, performing multiple independent diffusion trajectories to explore diverse binding geometries. Candidate backbones were filtered through key metrics and visual inspection. The best-scoring backbone was then optimized using ProteinMPNN to create seq.. Read More»
  

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