International Journal of Cancer Research & Therapy(IJCRT)
ISSN: 2476-2377 | DOI: 10.33140/IJCRT
Impact Factor: 1.382
The Effectivity of Artemisia Vulgaris Extract on Cyclin-D1 and Ki-67 Decreased as a Supplementation of Adenocarcinoma Mammae Chemotheraphy (Study on C3H Mice Given AC Chemotheraphy Regimen)
Abstract
Ahimsa A, Budijitno S and Handojo D
Background: The incidence of breast cancer worldwide is still high. Surgery remains the top choice with other modalities of chemotherapy, radiation, and suplementation such as Artemisia vulgaris (AV).
Aim: The study was aimed to demonstrate that administration of AV extract decreased the expression of Cyclin-D1 and the expression of Ki-67 in adenocarcinoma mammae.
Method: This study used “Posttest only control group design” on 24 females C3H mice that were randomly selected and divided into four groups: group K (control), P1 (chemotherapy), P2 (extract), and P3 (combination). Adenocarcinoma mammae comes from the inoculation of donor mice. Chemotherapy of Adriamycin 60 mg / m2 and Cyclophosphamide 600 mg / m2 were given in 2 cycles. AV 13 mg (0.2 ml) was given once daily orally. Cyclin-D1expression and Ki-67 expression were evaluated by imunohistochemical staining.
Result: Mean of Cyclin-D1expression and Ki-67 expression were found in groups K, P1, P2, P3 were 35,30 + 0,72; 20,38 + 0,67; 33,50 + 0,71; 17,36 + 0,66; and 66,44 + 0,65; 35,40 + 0,65; 64,12 + 0,85; 32,32 + 0,61. The statistical analysis showed that there were significant differences in the expression of Cyclin-D1 between groups of K vs P1, P3 (p = 0,001), P1 vs P2 (p = 0,001), P1 vs P3 (p = 0,045), P2 vs P3 (p = 0,001), and in Ki-67 expression between groups of K vs P1, P3 (p = 0,001), P1 vs P2 (p = 0,001), P1 vs P3 (p = 0,041), P2 vs P3 (p = 0,001). Correlation analysis between Cyclin-D1 expression and Ki-67 expression showed significant correlation (p = 0,030 dan r = 0,914).
Conclusion: Artemisia vulgaris is potential as supplementation that can improve the effectivity of AdriamycinCyclophosphamide chemotherapy in terms of decreased expression of Cyclin-D1 and expression of Ki-67 adenocarcinoma mammae of C3H mice.