Exogenous H2S Regulates CSE Expression in HUVECs under Hypoxic Conditions

Abstract

Maoxian Wang*

Hydrogen sulfide (H2 S) is a gaseous signaling molecule synthesized in the body by three enzymes: cystathionine-beta- synthase (CBS), cystathionine-gamma-lyase (CSE), and 3-mercaptopyruvate sulfurtransferase (3-MST). CSE plays a crucial role in the production H2 S in the cardiovascular system. The primary objective of this study was to investigate the effects of exogenous H2 S on CSE expression in vascular endothelial cells under hypoxic conditions. Using luciferase assays, quantitative RT-qPCR, and western blotting, we conducted a comprehensive analysis of the regulatory effects of 100 μM H2 S on CSE transcription and expression in human umbilical vein endothelial cells (HUVECs) subjected to hypoxia. This study specifically examined the impact of exogenous H2 S on CSE promoter activity in HUVECs. Notably, compared to 0 μM H2 S, exposure to 100 μM H2 S resulted in a significant reduction in mRNA and CSE protein expression in HUVECs following a two-hour hypoxic period. The administration of exogenous H2 S further diminished CSE expression at various time points in HUVECs under hypoxic conditions. Technical terms were defined based on their initial applications. These findings strongly suggest that vascular endothelial cells respond to fluctuations in blood H2 S levels during oxygen-deficient periods.

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