Dr S Sivakumar, Ph.D
Research Scientist, BIOTECHNOLOGY
Thiruvalluvar University, CHENNAI,TAMIL NADU, India
Biography
[2013-2020]
Doctor of Philosophy (Ph.D.) in Zoology (Aquaculture Biotechnology)
C. Abdul Hakeem College, Melvisharam , Thiruvalluvar University
Thesis Title: "Study on the New Strain of White Spot Syndrome Virus Isolated from Farm-Reared Litopenaeus vannamei in India"
Awarded on December 7, 2020
[2011-2013]
Master of Science (M.Sc.) in Zoology
C. Abdul Hakeem College, Melvisharam
[2010-2011]
Bachelor of Education (B.Ed.)
Meera College of Education, Sholinghur
[2007-2010]
Bachelor of Science (B.Sc.) in Zoology
C. Abdul Hakeem College, Melvisharam
Research Interest
Molecular Biology:
? Nucleic acid (DNA & RNA) and Plasmid extraction and purification
? Primer designing, PCR, RT-PCR, Real-time PCR
? Cloning and Construction of libraries in plasmid vectors
? Ultra Centrifuge Beckman
Microbiology:
? Isolation
? Identification
? Propagation of aquatic (shrimp and fish)
? Pure culture techniques
? Glycerol stock maintenance
? Bacterial cytotoxicity
Protein Biochemistry:
? SDS-PAGE
? Indirect ELISA and Sandwich ELISA
? Western Blot and Dot blotting
? Polyclonal antibodies
? Antibody conjugation
? Protein purification
? Formulation
? Protein estimation by Biochemical assays
? Immunological Assays
Bioprocess Technology:
? Culture handling (inoculum)
? Upstream Process (Fermentation Process)
? Downstream Process
? Recombinant protein and Probiotic strains
? Lyophilizer
? Sonicator
? Kjeldahl Instrument for Nitrogen analyzer
? Spray drying Technology
IgY Technology:
? Production
? Purification
? Therapeutic application of Egg Yolk Antibodies
Cell Culture:
? Development and characterization of cell lines
? Cryopreservation and retrieving of established cell lines
? Surveillance of viruses
? Virus infectivity and Titer studies (TCID50)
? Viral purification by Ultracentrifugation
? Gene-expression studies
? Toxicology and Genotoxicology
? Immune confocal microscopy
? FACS (Fluorescence-Activated Cell Sorting)
? Karyotyping
? Cell growth kinetics
Animal Maintenance:
? Maintenance of infected and uninfected fish, mice, shrimp, crab, rabbits, and hens.
