Journal of Veterinary Health Science(JVHS)
ISSN: 2831-3887 | DOI: 10.33140/JVHS
Impact Factor: 0.762
Samia Ahmed Kama
Professor Dr. of Virology, Animal Health Research Institute, Egypt
Publications
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Research Article
Lumpy Skin Virus; Herpesviridae Inevitable Synergism
Author(s): Samia Ahmed Kama*
Background: Lumpy skin virus (spp. Capripox) causes huge economic losses in farm animals. Herpesviridae spp. always showing synergistic role and worsen the disease symptoms. Negative staining of Transmission Electron Microscope techniques used in diagnosis to confirm the results for avoiding differential diagnosis doubts. Materials and Methods: Samples of Cutaneous crusts collected from infected cattle were tested by TEM and PCR (Conventional Polymerase Chain Reaction) then sequenced by ORF103 gene. Results: My study investigated LSD outbreak of non-vaccinated cattle. The confirmed diagnosis was outbreak of infection by Lumpy skin disease virus isolate Evros / GR/15 and 99% identity. A comparison of the gene sequence of the vaccine against the virus showed significant divergence between them. Electron microscopy re.. Read More»