Advances in Bioengineering and Biomedical Science Research(ABBSR)
ISSN: 2640-4133 | DOI: 10.33140/ABBSR
Impact Factor: 1.7
Nicholas Wolpert and Lauren Gollahon
Department of Biological Sciences, Texas Tech University, 2500 Broadway, Lubbock, Texas 79409, United States
Publications
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Research Article
A Novel Cell Culture Scratch Assay Platform Generates Reproducible Gaps for Quantitative Cell Movement-Based Studies
Author(s): Nicholas Wolpert and Lauren Gollahon*
Scratch assays are routinely performed for multiple research applications in cell biology (i.e., migration, metastasis, repair mechanisms, etc.). Conventional scratches are usually generated with pipette tips. User inconsistencies and varying pipette tips are major obstacles to reproducibility and quantitative power. Here, we present a novel, scratch/gap generating, cell culture plate-based, platform that addresses these issues, providing consistency and reproducibility across multiple users, significantly reducing variability, and increasing quantitative outcomes... Read More»