International Internal Medicine Journal(IIMJ)

Department of Pharmacy, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing, China

Publications

• Research Article   
  Endocytosis and Transport of Silica Nanoparticles in BeWo b30 Cells
  Author(s): Mengyao Wu, Weijie Jiang, Junyu He, Hongbo Tang*, Haibo He*, Jihong Zhang*, Hongwu Wang* and Hongwu Wang*
  
  An understanding of the transport of silica nanoparticles (NPs) across the placental barrier is important in perinatal medicine. The cytotoxicity of silica NPs was investigated in this study. In up-take assays, we examined the size of NPs, as well as the effects of various inhibitors, on the internalization of silica NPs in BeWo b30 cells. The expression levels of PI3K, AKT and GSK3β were assessed after the cells were treated with silica NPs and/or the PI3K/ AKT signaling pathway inhibitor LY294002. The integrity of the cell monolayer was assessed by culturing cells on Transwell inserts and measuring the transepithelial electrical resistance, assessing fluorescein sodium transport, and staining the tight junction protein zonula occludens-1. Silica NPs were spherical in shape, and concentrations <300 μg/mL were not cytotoxic. The internalization of silica NPs with .. Read More»
  

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