Chanwit Tribuddharat
Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Thailand
Publications
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Research Article
Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR Analysis as a Trace for Burkholderia Pseudomallei in Myanmar
Author(s): Nay Myo Aung*, Chanwit Tribuddharat, Narisara Chantratita, Khine Khine Su, Htet Wai Moe, Htin Linn Naing Soe and Khine Zaw Oo
Melioidosis is a potentially fatal disease caused by the bacterium Burkholderia pseudomallei, which is endemic in Southeast Asia, including Myanmar. The typeability of enterobacterial repetitive intergenic consensus (ERIC)-PCR was assessed for 21 B. pseudomallei by using the results of sequence types (STs) of multilocus sequence typing (MLST) method. Among 5 soil and 16 clinical B. pseudomallei isolates, it was seen that most of the major bands were quite similar in position but different in minor band formation. Therefore, ST 90 of two soil strains (Tontae_NMBP001 and Tontae_NMBP002) displayed the same ERIC banding pattern, while ST 56 of two clinical isolates (MMBP005 and MMBP010) exhibited a single type. Both of those two clusters were found to be the same ST in the MLST method. The shared group STs showed four or three satellite variants in the MLST scheme. One novel studied ST (S.. Read More»