International Internal Medicine Journal(IIMJ)

Department of Zoology D. A-V. College Chhatrapati Shahu Ji Maharaj University, India

Publications

• Research Article   
  Cytogenetic Analysis of Mitotic Cell Cycle Phases in Allium cepa Root Meristem: A Model for Chromosomal Behaviour and Environmental Toxicity Assessment
  Author(s): Shalini Gupta* and Anuj Kumar
  
  Onions (Allium cepa) are commonly employed in vitro to evaluate the effects of chemical agents. Onion chromosomes have long been prepared using the acetic orcein staining technique. However, aceto-orcein stain is corrosive and deadly since it contains oxidizing agents like organic peroxides, toxic compounds like cyanides, acid corrosives, and radioactive materials. This study examined the mitotic activity in the roots of onion plants to ascertain the impact of the aceto orcein dye's soaking period on actively dividing root cells. Using the aceto-orcein squash procedure, a number of root tips from each bulb were collected, soaked for 1, 3, and 24 hours in aceto-orcein stain, and then processed further for cytological analyses. The purpose of the research was to examine the impact of chromosomal aberration and mitotic index on onion roots. It will reveal how many cells are actually .. Read More»
  

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