The Evaluation of Possibility for Future Root Canal Irrigant Candidate by Catechin Solution
Abstract
Yoshishige Yamada, Yuichi Kimura, Tatsuya Shibata and Mozammal Hossain
To treatment for periapical periodontitis, root canal irrigation is one of most important process to obtain success of healing Sodium hypochlorite (NaOCl) is the most widely used as root canal irrigant. However using NaOCl on endodntics treatment should be considered its problems such as allergy, serious tissue damage when inadvertently injected into the maxillary sinus or oral mucosa. Therefore another root canal irrigant that is safety and having high antimicrobial effect is desirable. In this study, catechin was to evaluated microbicidal efficacy to the Enterococcus. faecali (E.faecalis) and considered the possibility to be the future root canal irrigant candidate. 10%(100mg/ml) to 30%(300mg/ml) of catechin solution were prepared and used for this study. The anti-microbiologic effect against E.faecalis was evaluated with different concentrations (low: 5x107 CFU/ml, high: 5x109 CFU/ml), and different reaction time like as from 5minutes to 30 minutes respectively. Then they were placed onto BHI agar plates and incubated for 1 week at 37ºC. Then the number of colony formation was counted. Prevent colony formation by 30% catechin solution was caused within 5minutes in low density of E.faecalis, and within 20 minutes in high density of it. 20% catechin solution leaded to no colony formation at 10 min (low density) and 30 min (high density), respectively. For 10% catechin solution, it was necessary to attain no colony formation should be 30 minutes (low density), however high density of E.faecalis was not killed completely within 30minutes. These results suggested that Catechin has a benefit to anti-microbiologicl effect against E.faecalis. However to determine Satisfactory anti-microbiological effect, high concentration of catechin solution and long reaction time should necessary. Therefore usage of catechin in future root canal treatment might be recommended not for candidate of root canal irrigant but for candidate of intracanal medication