Over Expression of Recombinant Staphylokinase and Reduction of Inclusion Bodies Using IPTG as Inducer in E. coli BL21 DE3

Abstract

Barakat Abdul Razzaq Mutar, Kiransinh N. Rajput, Vikram H. Raval, Rakesh Kumar R. Panchal

A previous research and studies have established the significance of describing Staphylokinase as a thrombolytic treatment, particularly in patients with cardiovascular disease, stroke, and other life-threatening disorders. Staphylokinase plays a significant part in the coagulation process through formation plasmin-Staphylokinase complex on the surface of the clot which activates plasminogen is generated by some strains of Staphylococcus aureus. The publication described the use of the E. coli strain BL12DE3 to produce the protein via the pET21a transporter is predicated on the use of a chemical stimulator (IPTG) which plays an important role in regulating protein recombinant by stimulating protein synthesis. Strain BL12DE3 activates T7 polymerase, encoding LacI via the pET21a transporter. It has been demonstrated that the IPTG inducer achieves the maximum level of protein expression in the shortest amount of time. A Staphylokinase was generated as a soluble protein in excess of 45 % by SDS-PAGE and subsequently purified by chromatography.

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