Isolation and Identification of Faecal Coliforms in Giri Stream Water

Abstract

Josiah Mohammed, Ogbu J.C., Mabawonku J.T., and Olarinoye B.M.

This study focused on the isolation and identification of faecal coliforms in the water of Giri Stream, situated in the Gwagwalada Area Council of the Federal Capital Territory (FCT), Abuja, Nigeria. A total of twenty (20) water samples were collected from ten designated points along the stream for analysis. The Multiple Tube Fermentation Technique was employed to assess the presence of fecal coliforms, revealing Most Probable Number (MPN) ranging from 92 to 300 MPN/100ml across the samples. All samples tested positive for lactose fermenters. Confirmatory tests indicated the presence of Escherichia coli through the formation of characteristic green metallic sheen on EMB agar. This is a key indicator of fecal pollution. The results emphasize the urgent need for regular monitoring of water quality and enhancements in sanitation practices to reduce health risks linked to contaminated water sources. Suggested actions include improving sanitation infrastructure, initiating educational campaigns to raise awareness about water quality issues, and fostering collaboration among local stakeholders to effectively tackle water quality challenges.

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