In Silico Investigation of Mapk1 Mediated Key Protein Interactors in Lung Squamous Cell Carcinoma
Abstract
Zafar Abbas Shah, Fareeha Ambreen and Rabea Ejaz
MAPK1 signaling pathway promotes development and survival of epithelial tissue. MAPKs family controls the activity of micro- environment by triggering the intracellular signaling. The focus of this study to understand the MAPK1 PPI by using STRING database and retrieve top twenty interaction network to analyze the significance of MAPK1 related proteins in lung Squamous cell carcinoma. We used cBioPortal a cancer platform to perform gene alteration analysis to uncover their participation in lung SqCC progression. We identified TP53 gene showed high ratio alteration among eleven lung SqCC linked disease causing MAPK1 mediated pathway genes in comparative study of lung squamous cell carcinoma TCGA, nature 2012 dataset. In pres- ent study by meta-analysis we characterized vital MAPK1 signaling pathway altered interactions that are MYLK, BCL2, TP53, BCL6, RAF1, CTTN, FGF19, FGF3, FGF4, ATR, TP63, HES1, CDKN2A, RASA, MRAS, TNFSF10, IL12A, TEK, PTEN, AGTR1, DVL3, EPHB1, TRIO, RPS6KA1, RPS6KA2, FOS, FGFR1, PAK2, PRKCI, PIK3CA, PIK3CG and KDR that involved in cell proliferation, growth, differentiation, tumor suppressed activities and apoptosis. In aberrant conditions they act as oncogenic and anti-apoptotic agents. The empirical validation of MAPK1 signaling cascade key interactions in lung SqCC in future that gives fruitful selection of therapeutic targets for squamous cell carcinoma.