Highly Differentiated Human Airway Epithelial Cells Immunological Response to Respiratory Syncytial Virus and Human Rhinovirus C

Abstract

Tshetiz Dahal, Pramod Singh Tanwar

Background: Highly differentiated human airway epithelial cells immunological response to respiratory syncytial virus and human rhinovirus C.

Methods: HBE cells were developed in an ALI culture before being infected with two RSV isolates, the fulllength cDNA clones of HRV-C651 and HRV-C15, two clinical isolates of HRV-C79, and HRV-C101. On cytokine secretion, the impact of HRV-C was evaluated and contrasted with that of RSV.

Results: HRV-Cs infect and propagate in fully differentiated HBE cells and significantly increase the secretion of IFN-λ1, CCL5, IP10, IL-6, IL-8, and MCP-1. The virus loads positively correlated with the levels of the cytokines. HRV-C induced lower secretion of CCL5 (P=0.048), IL-6 (P=0.016), MCP-1 (P=0.008), and IL-8 (P=0.032), and similar secretion of IP10 (P=0.214) and IFN-λ1 (P=0.214) when compared with RSV.

Conclusion: Infection and multiplication of HRV-C were promoted by the HBE ALI culture system, and HRV-C generated substantially weaker cytokine production than RSV

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