Comparison of Porous Graphitic Carbon (Pgc), Hydrophilic Interaction Liquid Chromatography (Hilic) And Reversed-Phase (Rp) Columns

Abstract

Oluwaseun Olanike Ajayi & Victor Oluwatosin Ajayi

The purpose of this review was to compare porous graphitic carbon (PGC), hydrophilic interaction liquid chromatography (HILIC) and reversed-phase (RP) columns on the basis of stationary phase, interaction mechanism, analyte types (suitable analytes) and application. PGC, HILIC, and RP columns are three common types of liquid chromatography methods used for the separation of various analytes based on different properties. Each column has a distinct mechanism of retention, making them suitable for different applications. PGC columns use a unique graphitic surface to retain polar and non-polar compounds, while HILIC columns separate highly polar analytes using a hydrophilic stationary phase and a high-organic-content mobile phase. RP columns, use a non-polar stationary phase to retain non-polar compounds from polar mobile phases. The paper also highlighted some challenges with PGC, HILIC and RP Columns.

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