Anti-Fungal Effects 0f Ambon Banana Skin Extract (Musa paradisiaca Linn. Var. Sapientum) on Candida albicans ATCC 10231 (In Vitro)
Abstract
Minasari Nasution, Yendriwati and Luqman Ghani Adlan Hasibuan
Introduction: Disease is a global problem for health. One of the most common infectious diseases is a disease caused by fungi (mycosis), a species of Candida (candidiasis) caused by Candida albicans. These bananas are generally often consumed daily by Indonesians. Ambon banana skin extract (Musa Paradisiaca Linn. Var.Sapientum) is a nutritious medicinal plant, because Ambon banana skin waste has active antifungal compounds, namely tannins, flavonoids, quinones, phenols, and steroids. Which can damage fungal cell wall membrane proteins, damage the DNA chain causing brittle cell walls resulting in fungal cell death.
Objectives: Research to determine the zone of inhibition, MIC and MBC of Ambon banana skin extract at concentrations of 100%, 90%, 80%, 70%, 60%, and 50% against Candida albicans ATCC® 10231™.
Method: Laboratory experimental research with a post test only control group research design, the sample used was 18 samples, tested with the Anova test. In determining the inhibition zone using the well diffusion method, the measurement of the inhibition zone uses the calipers, MIC and MBC using the dilution method. Ambon banana skin was extracted by maceration method using 70% ethanol solvent, carried out 6 treatments with various concentrations. Each treatment was repeated 3 times.
Results: The inhibition zone research at concentrations of 100%, 90%, 80%, 70%, 60%, and 50% with an average of 13.2mm 11.6mm 11.3mm, 10.5mm, 9.9mm, 8.3mm. The MIC of each concentration was clear and equalized according to the standard of 0.5 McFarland (1.5 x 108 CFU / ml) with potato dextrose broth medium, and the MBC was emphasized with potato dextrose agar (PDA) medium. Conclusion Inhibition zone concentrations of 100% -70% indicate strong criteria, 60-50% including weak criteria. MIC obtained at a concentration of 50%, MBC at a concentration of 60%. In this study, using a concentration of 70% indicates an average diameter of above 10mm, meaning that it is in accordance with the David and Stout method which states that the criteria are strong at an average of 10-20 mm. a concentration of 70% is used because it is effective in killing Candida albicans ATCC® 10231™, 100% -80% has a high toxicity which can cause toxic effects on all organisms such as the body, fungi, plants, etc.