International Journal of Clinical & Experimental Dermatology(IJCED)
ISSN: 2476-2415 | DOI: 10.33140/IJCED
Impact Factor: 1.9
The effects of different stimulated macrophages on HaCaT proliferation and secretion.
Abstract
Yanyan Feng, Jingying Sun, Junfeng Yu, Jianxia Chen, Jianyong Liu, Xinmei Liu, Zhengqun Wong, Xiaojing Kang
Background-As an important cell of innate immunity, macrophages secrete a large number of inflammatory cytokines, including TNF-α, which may play crucial role in the initiation stage of psoriatic inflammation. Keratinocytes are the effector cells of psoriasis and may produce circulatory stimulation networks with other immune cells in the lesions. Understanding the effect of macrophages induce keratinocytes’ biological function is necessary to understand the mechanisms of psoriasis.
Objectives-To evaluate the effects of different polarized macrophages on the proliferation, differentiation, apoptosis and secretion of immortalized human keratinocytes (HaCaT). Materials and Methods-Establish co-culture system of macrophages and HaCaT in vitro to mimic macrophage infiltration microenvironment of psoriasis skin. The proliferation of HaCaT was detectd by CCK8, Cell cycle. Apoptosis of HaCaT was analysed by flow cytometry. The secretion of IL-1β, TNF and IL-6 by HaCaT was analysed by ELISA.
Results-The results showed a decreased proliferation of HaCaT when co-cultured with LPS-induced macrophage and upregulated IL-6, IL-1β and TNF-α. While more HaCaT cells were blocked in the G0/G1 phase, the number of mitotic phase cells decreased and apoptotic cells increased. On the contrast, HaCaT showed increased proliferation when co-cultured with IL-4-induced macrophage, while no difference in apotosis of HaCaT. Moreover, proinflammatory cytokine TNF-α and IL-12 production by LPS-induced macrophage were significantly increased.
Conclusion-Considering the changes in the biological function of HaCaT cells caused by the infiltration microenvironment of macrophages, these findings indicate that the polarization process of macrophage is a crucial mechanism in the development of psoriasis, and the underlying immunological mechanisms should be understood.