Hepatotoxic Manifestations of Arsenic Trioxide Loaded Poly (Lactide-Co-Glycolide) Nanoparticles in Wistar Rat

Abstract

Yeshvandra Verma and S V S Rana

Present study reports on the hepatotoxic manifestations of arsenic trioxide loaded poly lactide-co-glycolide nanoparticles (As2 O3 -PLGA- NPs) in rats. As2 O3 -PLGA- NPs enhances the activity of serum transaminases. As2 O3 - PLGA-NPs are potential inducer of lipid peroxidation in mitochondria as well as microsomes. Mitochondrial lipid peroxidation was higher than the microsomal lipid peroxidation. CYP450 2E1 was lower in the liver of As2 O3 -PLGA- NPs treated rats in comparison to arsenic trioxide treated rats. GSH showed lower values than control rats and arsenic trioxide treated rats. Glutathione-S-transferase inhibited by arsenic trioxide, non significant increase was recorded in the liver of As2 O3 -PLGA- NPs treated rats. As2 O3 -PLGA- NPs readily accumulates in liver and induces peculiar histopathological changes viz. intracytoplasmic/intranuclear inclusions and apoptosis. Since As2 O3 -PLGA- NPs are being considered as a suiTable chemo-preventive agent against different types of cancer, its toxicological properties are of prime concern from bio-safety point of view. Thus, present observations seem to be important from human health risk assessment point of view.

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