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YPD Medium

YEPD or yeast extract peptone dextrose, also often abbreviated as YPD, is a complete medium for yeast growth. It contains yeast extract, peptone, double-distilled water, and glucose or dextrose. It can be used as a solid medium by including agar. The yeast extract will typically contain all the amino acids necessary for growth. By being a complete medium, YEPD cannot be used as a selection medium to test for auxotrophs. Instead, YEPD is used as a growth medium to grow yeast cultures. YEPD typically contains 1% yeast extract, 2% peptone, and 2% glucose/dextrose in distilled water. It may be made as a broth or made into an agar gel by adding 1.5 to 2% of agar to hot broth and allowing the mixture to cool and solidify.

Last Updated on: Jun 17, 2025

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