The Epstein-Barr virus (EBV) is an important human pathogen that is associated with numerous cancers. The major oncoprotein of the virus, Latent Membrane Protein 1 (LMP1), is essential for EBV B4-cell immortalization and is sufficient to transform rodent fibroblasts. This viral transmembrane protein activates multiple cellular signaling pathways by engaging critical effector molecules and thus acting as a ligand-independent growth factor receptor. LMP1 also traffics to various sub-cellular compartments that are likely important for its signaling capacity and transforming abilities. The study of LMP1 signaling and protein-protein interactions has provided valuable insight into the molecular details of EBV-induced carcinogenesis and cellular signal transduction. This review will summarize these previous findings and focus on modern proteomics based methods, particularly 2D difference ingel electrophoresis (DIGE), in the discovery of novel signaling pathways and molecules affected by LMP1.Scholarly peer review is the process of subjecting an author's scholarly work, research, or ideas to the scrutiny of others who are experts in the same field, before a paper describing this work is published in a journal. The work may be accepted, considered acceptable with revisions, or rejected. Peer review requires a community of experts in a given (narrowly defined) field, who are qualified and able to perform reasonably impartial review.