Cyclodextrin glycosyl transferase (CGTase, EC 2.4.1.19) is an important industrial enzyme, unique in its ability to convert starch and related glycans into non-reducing, cyclic malto-oligosacchrarides called cyclodextrins (CDs) via a cyclization reaction, an intramolecular transglycosylation reaction . Moreover, it is an important hydrolytic enzyme that carries out reversible intermolecular coupling and disproportionation of maltooligosaccharides . CDs are non-reducing cyclic structures consisting mainly of 6, 7 or 8 glucose residues, joined by α-(1,4) linkages, for α-, β- and γ CD cyclodextrin, respectively. The arrangement of glucose units in the CD molecule results in the shape of a hollow truncated cone with a hydrophilic external surface, which makes CDs water-soluble, and a hydrophobic internal cavity, which enables CDs to accommodate and form inclusion complexes with various organic and inorganic compounds . The profound advantageous effects on the guest molecules after formation of inclusion complexes with cyclodextrins, has led to many applications of cyclodextrins in various areas including textile, pharmaceuticals, bioconversion, cosmetics, environmental protection, and food industries